metal affinity chromatography plate (TaKaRa)
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TaKaRa
metal affinity chromatography plate
Metal Affinity Chromatography Plate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metal+affinity+chromatography/Capturem+His-Tagged+Purification+24-Well+Plate/bio_rxiv__64898__2026__04__27__720530-400-17-26
Average 99 stars, based on 7 article reviews
Metal Affinity Chromatography Plate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metal+affinity+chromatography/Capturem+His-Tagged+Purification+24-Well+Plate/bio_rxiv__64898__2026__04__27__720530-400-17-26
Average 99 stars, based on 7 article reviews
metal affinity chromatography plate - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Multi-dimensional optimization of a lysin towards a ribolysin against life-threatening S. aureus infections: Fc-LysM-CHAP and its strong synergy with standard of care antibiotics"
Article Title: Multi-dimensional optimization of a lysin towards a ribolysin against life-threatening S. aureus infections: Fc-LysM-CHAP and its strong synergy with standard of care antibiotics
Journal: bioRxiv
doi: 10.64898/2026.04.27.720530
Figure Legend Snippet: a.) Purification of Fc-hel8-L1-3. Fc-hel8-L1-3 was purified from Expi293 supernatants by Ni-NTA affinity chromatography, followed by removal of the purification tag, protein concentration and buffer exchange. Samples of culture supernatants at 24 h and 72 h post transfection, Ni-NTA eluates and samples after dialysis were taken and analyzed by SDS-PAGE under reducing and (where indicated) non-reducing conditions followed by Coomassie staining. b.) SEC behavior of Fc-hel8-L1-3. Dialyzed samples of purified Fc-hel8-L1-3 were analyzed by size exclusion chromatography (SEC) using a Superdex 200 increase 10/300 gl column. Protein absorbance at 280nm was normalized against the maximum signal recorded during the SEC run. The void volume of the column at ∼8 mL is indicated. c.) Impact of protein charge on PK and MIC of different Fc-LysM-CHAP variants. AUC at 4 h (AUC 4h , blue) was determined from the PK curves shown in . MIC values (red) are as determined in . AUC 4h (log10 scale) and MIC (log2 scale) were plotted against the charge at pH7 of each Fc-LysM-CHAP variant (as determined from the amino acid sequence). The relationship between charge and AUC was tested for linear correlation with the Pearson method; the relationship between charge and MIC was tested for non-linear correlation with the Spearman method (correlation coefficients (r) and significance values (P) are indicated). AUC: area under curve; MIC: minimal inhibiting concentration. d.) PK comparison of dimeric and monomeric Fc fusions. PK curves of dimeric (red) and monomeric (green) formats of Fc-hel8-L1 and the corresponding naked lysin (L1, blue) were determined as described in .). Datapoints represent mean and standard deviation (n=3).
Techniques Used: Purification, Affinity Chromatography, Protein Concentration, Buffer Exchange, Transfection, SDS Page, Staining, Size-exclusion Chromatography, Variant Assay, Sequencing, Concentration Assay, Comparison, Standard Deviation
Related Articles
Affinity Chromatography:Article Title: Lipid-Modulated, Graduated Inhibition of N-Glycosylation Pathway Priming Suggests Wide Tolerance of ER Proteostasis to Stress Article Snippet: .. Immobilized Article Title: BRD4 binds the nucleosome via both histone and DNA interactions Article Snippet: .. HIS-tagged BRD4 was expressed in E. coli BL21(DE3)pLysS cells through IPTG induction or autoinduction at 23°C and purified from crude lysates via Article Title: Force-Triggered Thermodynamically Uphill Disulfide Reduction through Sulfur Oxidation State Control Article Snippet: .. The proteins from the lysate were purified twice, first by Article Title: Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O -glycans to completion Article Snippet: .. Recombinant His-tagged protein was purified from cell-free extracts using immobilized Article Title: Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219. Article Snippet: The Fab fragment of anti-osteocalcin C-terminal peptide antibody KTM219 was expressed in E. coli SHuffle T7 Express lysY (New England BioLabs, Ipswich, MA, USA) with a protein expression plasmid pET-Fab (KTM219) [26] as previously described [7,26]. .. The His6-tagged proteins were purified using immobilized Article Title: Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219 Article Snippet: The Fab fragment of anti-osteocalcin C-terminal peptide antibody KTM219 was expressed in E. coli SHuffle T7 Express lysY (New England BioLabs, Ipswich, MA, USA) with a protein expression plasmid pET-Fab (KTM219) [ ] as previously described [ , ]. .. The His 6 -tagged proteins were purified using immobilized Binding Assay:Article Title: Lipid-Modulated, Graduated Inhibition of N-Glycosylation Pathway Priming Suggests Wide Tolerance of ER Proteostasis to Stress Article Snippet: .. Immobilized Western Blot:Article Title: Lipid-Modulated, Graduated Inhibition of N-Glycosylation Pathway Priming Suggests Wide Tolerance of ER Proteostasis to Stress Article Snippet: .. Immobilized Purification:Article Title: PELP1 coordinates the modular assembly and enzymatic activity of the rixosome complex Article Snippet: .. Cleared lysates were purified using Article Title: BRD4 binds the nucleosome via both histone and DNA interactions Article Snippet: .. HIS-tagged BRD4 was expressed in E. coli BL21(DE3)pLysS cells through IPTG induction or autoinduction at 23°C and purified from crude lysates via Article Title: Force-Triggered Thermodynamically Uphill Disulfide Reduction through Sulfur Oxidation State Control Article Snippet: .. The proteins from the lysate were purified twice, first by Article Title: Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O -glycans to completion Article Snippet: .. Recombinant His-tagged protein was purified from cell-free extracts using immobilized Article Title: Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219. Article Snippet: The Fab fragment of anti-osteocalcin C-terminal peptide antibody KTM219 was expressed in E. coli SHuffle T7 Express lysY (New England BioLabs, Ipswich, MA, USA) with a protein expression plasmid pET-Fab (KTM219) [26] as previously described [7,26]. .. The His6-tagged proteins were purified using immobilized Article Title: Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219 Article Snippet: The Fab fragment of anti-osteocalcin C-terminal peptide antibody KTM219 was expressed in E. coli SHuffle T7 Express lysY (New England BioLabs, Ipswich, MA, USA) with a protein expression plasmid pET-Fab (KTM219) [ ] as previously described [ , ]. .. The His 6 -tagged proteins were purified using immobilized Chromatography:Article Title: PELP1 coordinates the modular assembly and enzymatic activity of the rixosome complex Article Snippet: .. Cleared lysates were purified using other:Article Title: Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O-glycans to completion. Article Snippet: PGMIII (Sigma) was prepared by dissolving it overnight in sterile deionized water. Recombinant:Article Title: Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O -glycans to completion Article Snippet: .. Recombinant His-tagged protein was purified from cell-free extracts using immobilized |